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2nd day in laboratory
Daily Report – Day 2 at Dr. B Lal Laboratory
Date: February 11, 2025
Day: Tuesday
Introduction
My second day at Dr. B Lal Laboratory was filled with learning experiences and interactions with senior professionals and classmates. Today, I had the opportunity to observe laboratory tests, understand essential instruments, and learn about incubation techniques.
Morning Session
Meeting with Mentors and Classmates
- Dr. Ola Sir: I met Dr. Ola Sir again, and he guided me regarding the structured workflow of the lab.
- Dr. Sandeep Sir: He emphasized the importance of making a daily report based on my observations and study material. He advised that keeping structured notes would help in understanding laboratory procedures more effectively.
- Vishal (Friend): We worked together and shared our understanding of different tests.
- Tanisha & Manvi (Classmates): It was great to see familiar faces, and we discussed our initial experiences in the lab.
Key Learnings & Observations
1. Respiratory Viral Panel & Disease Testing
- Observed testing for H1N1 (Swine Flu), Pneumonia Respiratory Panel, and other respiratory viral infections.
- Understood how molecular testing helps detect viral RNA and DNA in patient samples.
- Learned that these tests play a crucial role in diagnosing and treating respiratory infections efficiently.
2. Allergy Testing – Observation with Vishal
- Watched the multiple allergy tests performed to identify allergens that trigger reactions in patients.
- Understood the process of skin prick tests and blood allergy panels.
- Discussed with Vishal how different IgE levels indicate allergic responses.
3. Understanding Laboratory Equipment
Graduated Pipette & Its Uses
- Observed the correct technique for using a graduated pipette, which is essential for measuring and transferring precise liquid volumes.
- Learned that proper handling is crucial to maintain the accuracy of diagnostic tests.
Incubation Process
- Understood how incubation is used to grow microbial cultures.
- Learned about different incubation temperatures and durations for bacterial and viral cultures.
- Observed how agar plates are placed inside the incubator to allow microbial growth for further analysis.
End of the Day Reflection
Today’s experience reinforced the importance of precision, observation, and documentation in laboratory work. The guidance from senior doctors, the support from classmates, and hands-on experience with equipment helped me grasp key concepts more effectively.
I look forward to deepening my knowledge and improving my skills in diagnostic testing in the coming days.
Key Takeaways for Tomorrow
- Study and understand more about microbial culture techniques.
- Observe and learn about automated diagnostic machines.
- Start working on a structured daily learning journal as advised by Dr. Sandeep Sir.
This journey is just beginning, and each day brings valuable learning opportunities.

First Day in laboratory
A New Beginning at Dr. B Lal Laboratory
Today marked a significant step in my journey as I officially joined Dr. B Lal Laboratory on February 10, 2025. Excitement and nervousness blended together as I entered the building, ready to embark on a new learning experience.
My first interaction was with Ola Sir, who greeted me warmly and guided me through the initial process. After a brief introduction, he directed me to Charul Ma’am, who would oversee my onboarding. I made my way to the 2nd floor, where she welcomed me with a professional yet friendly demeanor.
She handed me the joining form, a crucial step in making my association official. As I filled it out, I reflected on how this opportunity aligned with my medical laboratory studies and future goals.
Once the paperwork was complete, I received a report booklet—a treasure trove of knowledge about laboratory procedures and diseases. This booklet symbolized the vast learning that awaited me, a bridge between theoretical knowledge and real-world application.
As I left the office, booklet in hand, I felt a sense of accomplishment and eagerness. Today was just the beginning, and I was ready to embrace the challenges and growth that lay ahead in this new phase of my career.
Story of Life
First Day in laboratory


Photoreceptor Cells: Rods and Cones
The human eye contains specialized cells in the retina called photoreceptors, which convert light into electrical signals that the brain interprets as vision. There are two main types of photoreceptor cells:
1. Rods
Rods are responsible for night vision and help detect objects in low-light conditions.
Characteristics of Rods:
- Highly sensitive to dim light (scotopic vision).
- Cannot detect colors (only shades of gray).
- More numerous than cones (about 120 million rods in each eye).
- Concentrated in the peripheral retina, helping with peripheral vision.
Pigment in Rods:
- Rhodopsin (Visual Purple) – A light-sensitive pigment that enables vision in the dark.
- Rhodopsin breaks down in bright light, making rods ineffective in daylight.
2. Cones
Cones are responsible for color vision and function best in bright light conditions.
Characteristics of Cones:
- Provide sharp, detailed vision (photopic vision).
- Detect colors (trichromatic vision).
- Fewer in number compared to rods (about 6 million cones in each eye).
- Concentrated in the central retina, especially in the fovea for high-acuity vision.
Pigments in Cones:
There are three types of cones, each with a specific pigment that responds to different wavelengths of light:
- Erythrolabe (Red-sensitive cones) – Detects long wavelengths (~565 nm).
- Chlorolabe (Green-sensitive cones) – Detects medium wavelengths (~535 nm).
- Cyanolabe (Blue-sensitive cones) – Detects short wavelengths (~420 nm).
Together, these three cone types allow the brain to perceive a full spectrum of colors through a process called color mixing.
Key Differences Between Rods and Cones
Feature | Rods | Cones |
---|---|---|
Function | Night vision | Color vision |
Light Sensitivity | High (works in dim light) | Low (needs bright light) |
Color Detection | No (black and white) | Yes (RGB colors) |
Number | ~120 million | ~6 million |
Location | Peripheral retina | Central retina (fovea) |
Pigment | Rhodopsin | Erythrolabe, Chlorolabe, Cyanolabe |
Understanding the roles of rods and cones helps explain conditions like color blindness, which occurs when certain cones are missing or not functioning properly.
For more educational resources, visit Saeed Ur Rahman.

Packed Cell Volume (PCV)
Introduction
Packed Cell Volume (PCV), also known as hematocrit, is a vital parameter in hematology. It refers to the proportion of red blood cells (RBCs) in the blood, expressed as a percentage of the total blood volume. PCV is a simple, yet crucial test for evaluating the oxygen-carrying capacity of the blood and diagnosing various hematological conditions.
This lecture will cover the definition, clinical significance, methods of determination, procedures, normal values, and interpretation of PCV.
I. Definition of Packed Cell Volume (PCV)
- PCV is the percentage of blood volume occupied by red blood cells after centrifugation.
- It is a measure of the relative proportion of cellular components to plasma in blood.
II. Clinical Significance of PCV
- Assessment of Anemia:
- Decreased PCV indicates anemia, reflecting reduced RBC levels.
- Polycythemia Diagnosis:
- Elevated PCV is a hallmark of polycythemia, indicating increased RBC concentration.
- Dehydration:
- Higher PCV can result from reduced plasma volume in dehydration.
- Blood Loss:
- PCV helps assess the severity of acute or chronic blood loss.
- Hydration Status:
- Changes in PCV can indicate overhydration or dehydration.
- Monitoring Therapy:
- PCV is used to monitor the effectiveness of treatments like blood transfusion or fluid therapy.
III. Methods for Determining PCV
1. Microhematocrit Method
- Most commonly used method due to its simplicity and speed.
2. Wintrobe Method
- A more traditional method using a Wintrobe tube.
3. Automated Hematology Analyzers
- Provide highly accurate and precise PCV measurements as part of a complete blood count (CBC).
IV. Microhematocrit Method in Detail
1. Principle
- Blood is centrifuged in a capillary tube, causing its components to separate into three distinct layers:
- RBCs: Settle at the bottom due to their higher density.
- Buffy Coat: Thin middle layer containing WBCs and platelets.
- Plasma: Topmost layer.
2. Equipment and Materials
- Microhematocrit centrifuge.
- Capillary tubes (heparinized for capillary blood; plain for venous blood).
- Sealant (e.g., clay or plastic caps).
- Microhematocrit reader or scale.
3. Procedure
- Collect blood:
- Capillary blood using a finger prick.
- Venous blood with EDTA as an anticoagulant.
- Fill the capillary tube to about 75% of its length.
- Seal one end of the tube with sealant.
- Place the tube in a microhematocrit centrifuge, ensuring it is balanced.
- Centrifuge at 10,000–12,000 rpm for 3–5 minutes.
- Measure the PCV using a hematocrit reader:
- Align the top of the plasma layer with the scale.
- Record the height of the RBC layer as a percentage of the total blood volume.
4. Advantages
- Quick and cost-effective.
- Requires minimal equipment.
5. Limitations
- May not distinguish between true RBC volume and trapped plasma.
- Not suitable for highly automated settings.
V. Wintrobe Method in Detail
1. Principle
- Blood is filled into a Wintrobe tube and centrifuged at a lower speed compared to the microhematocrit method.
2. Procedure
- Fill the Wintrobe tube with anticoagulated blood.
- Centrifuge at 2,300 rpm for 30 minutes.
- Read the height of the RBC column directly from the calibrated tube.
3. Advantages
- Can estimate trapped plasma volume.
- Provides detailed information on buffy coat size.
4. Limitations
- Time-consuming and less commonly used.
VI. Interpretation of PCV Results
1. Normal Values
- Men: 42–52%
- Women: 37–47%
- Children: 35–45%
- Newborns: 50–62%
2. Increased PCV
- Causes:
- Polycythemia vera.
- Dehydration.
- Chronic hypoxia (e.g., in COPD, high altitude).
- Implications:
- Increased blood viscosity.
- Higher risk of thrombosis.
3. Decreased PCV
- Causes:
- Anemia (nutritional, hemolytic, or aplastic).
- Chronic disease.
- Acute or chronic blood loss.
- Overhydration or pregnancy.
- Implications:
- Reduced oxygen-carrying capacity.
- Fatigue and shortness of breath.
VII. Sources of Error in PCV Measurement
- Inadequate mixing of blood.
- Improper sealing of capillary tubes.
- Incorrect centrifugation time or speed.
- Overanticoagulation leading to RBC shrinkage.
- Misreading due to poorly calibrated hematocrit readers.
VIII. Comparison of Methods
Method | Advantages | Disadvantages |
---|---|---|
Microhematocrit | Quick, cost-effective | Plasma trapping potential. |
Wintrobe | Measures buffy coat size | Time-consuming. |
Automated Analyzers | Highly accurate and reliable | Expensive equipment. |
IX. Clinical Applications
- Routine Health Checkups:
- PCV is a standard parameter in complete blood count (CBC) tests.
- Diagnosis and Monitoring:
- Anemia, polycythemia, and hydration status.
- Therapeutic Monitoring:
- Evaluating response to treatments like transfusion or rehydration therapy.
- Emergency Settings:
- Quick assessment of blood loss or dehydration.
X. Conclusion
Packed Cell Volume is a fundamental test in hematology that provides essential information about a patient’s red cell mass and overall blood composition. While simple, it plays a crucial role in diagnosing and managing a variety of hematological and systemic conditions.
Would you like additional details on clinical correlations or practical demonstrations?

Cardiovascular System
Here is a detailed lecture on the Cardiovascular System, with embedded links to relevant topics:
The Cardiovascular System
The Cardiovascular System, also known as the circulatory system, is a complex network responsible for transporting blood, nutrients, oxygen, hormones, and waste products throughout the body. It is vital for maintaining homeostasis, delivering essential substances to tissues, and removing metabolic waste.
Components of the Cardiovascular System
1. The Heart
The Heart is a muscular organ located in the thoracic cavity. It serves as the central pump of the cardiovascular system.
- Structure: The heart has four chambers: two atria (upper chambers) and two ventricles (lower chambers).
- Function: The heart pumps oxygen-rich blood to the body and oxygen-depleted blood to the lungs for reoxygenation.
- Layers: The heart consists of three layers:
- Epicardium: Outer layer.
- Myocardium: Muscular middle layer responsible for contractions.
- Endocardium: Inner lining of the heart.
2. Blood Vessels
The Blood Vessels form a closed network that transports blood. They include:
- Arteries: Carry oxygen-rich blood away from the heart to the body.
- Veins: Return oxygen-depleted blood to the heart.
- Capillaries: Thin, tiny vessels where the exchange of oxygen, nutrients, and waste products occurs between blood and tissues.
3. Blood
Blood is the fluid that circulates through the cardiovascular system, performing vital functions such as oxygen transport, nutrient delivery, and waste removal.
- Components of Blood:
- Red Blood Cells: Contain hemoglobin to carry oxygen.
- White Blood Cells: Fight infections and provide immunity.
- Platelets: Help in blood clotting.
- Plasma: The liquid portion that carries hormones, nutrients, and waste products.
Functions of the Cardiovascular System
- Transportation: Delivers oxygen, nutrients, and hormones to cells while removing carbon dioxide and other waste products.
- Regulation: Maintains body temperature, pH balance, and fluid balance.
- Protection: Through white blood cells, platelets, and plasma proteins, the cardiovascular system defends against infections and aids in wound healing.
The Circulatory Pathways
-
Systemic Circulation
- Systemic Circulation involves the movement of oxygen-rich blood from the left ventricle of the heart to the body and the return of oxygen-depleted blood to the right atrium.
-
Pulmonary Circulation
- Pulmonary Circulation involves the transport of oxygen-depleted blood from the right ventricle to the lungs for oxygenation and the return of oxygen-rich blood to the left atrium.
Heart Function and Cardiac Cycle
1. Cardiac Cycle
The Cardiac Cycle includes all the events in one heartbeat, divided into:
- Systole: Contraction phase where blood is pumped out of the heart.
- Diastole: Relaxation phase where the heart fills with blood.
2. Heartbeat Regulation
The Heartbeat is controlled by the conduction system, including:
- SA Node: The natural pacemaker initiating the heartbeat.
- AV Node: Delays the signal to allow ventricular filling.
- Bundle of His and Purkinje Fibers: Ensure coordinated contraction of the ventricles.
Common Cardiovascular Disorders
- Atherosclerosis: Hardening and narrowing of arteries due to plaque buildup.
- Hypertension (High Blood Pressure): Elevated blood pressure damaging blood vessels.
- Heart Attack: Blockage of blood flow to the heart muscle.
- Stroke: Disruption of blood supply to the brain.
- Arrhythmia: Irregular heart rhythms affecting blood flow.
Importance of Cardiovascular Health
Maintaining a healthy cardiovascular system is vital for overall well-being. This can be achieved through:
- Regular exercise.
- A balanced diet rich in fruits, vegetables, and low-fat proteins.
- Avoiding smoking and excessive alcohol consumption.
- Monitoring blood pressure, cholesterol, and glucose levels.
Conclusion
The Cardiovascular System plays an indispensable role in sustaining life by ensuring efficient blood circulation, nutrient delivery, and waste removal. Understanding its structure and functions is crucial for promoting cardiovascular health and preventing related disorders.

Human Respiratory System
Here is a detailed lecture on the Human Respiratory System, complete with relevant links:
Introduction: The Human Respiratory System
The Human Respiratory System is a biological system that enables the exchange of gases—primarily oxygen and carbon dioxide—between the body and the external environment. This process is essential for cellular respiration, where energy is produced. The respiratory system is composed of organs and tissues that work together to help humans breathe, and it plays a vital role in maintaining homeostasis.
1. Components of the Human Respiratory System
The respiratory system can be divided into the Upper Respiratory Tract and the Lower Respiratory Tract.
A. Upper Respiratory Tract:
-
Nose and Nasal Cavity:
- Function: Filters, warms, and moistens the incoming air.
- Structure: Lined with mucus and cilia to trap dust and microbes.
- Nasal Cavity plays a crucial role in smell perception and conditioning the air before it enters the lungs.
-
Pharynx (Throat):
- Function: A passageway for air and food.
- Divided into three parts: nasopharynx, oropharynx, and laryngopharynx.
- Pharynx connects the nasal cavity to the larynx and esophagus.
-
Larynx (Voice Box):
- Function: Produces sound and prevents food from entering the trachea using the epiglottis.
- Larynx is located below the pharynx.
B. Lower Respiratory Tract:
-
Trachea (Windpipe):
- Structure: A tube supported by C-shaped cartilage rings.
- Function: Transports air to the lungs and traps particles in its mucous lining.
- Trachea ensures an open airway to the lungs.
-
Bronchi and Bronchioles:
- Structure: The trachea divides into two bronchi, which further branch into smaller bronchioles.
- Function: Distribute air evenly within the lungs.
- Bronchi and Bronchioles facilitate airflow into the alveoli.
-
Lungs:
- Structure: The lungs are paired organs located in the thoracic cavity.
- Function: The primary site of gas exchange.
- Lungs contain millions of alveoli, where oxygen enters the blood and carbon dioxide exits.
-
Alveoli:
- Structure: Tiny air sacs surrounded by capillaries.
- Function: The site of gas exchange through diffusion.
- Alveoli are highly efficient due to their large surface area and thin walls.
2. Process of Breathing (Ventilation)
The process of breathing includes two main phases:
-
Inhalation (Inspiration):
- The diaphragm contracts and moves downward.
- The ribcage expands, creating negative pressure, which pulls air into the lungs.
- Inhalation brings oxygen into the lungs.
-
Exhalation (Expiration):
- The diaphragm relaxes and moves upward.
- The ribcage contracts, pushing air out of the lungs.
- Exhalation removes carbon dioxide from the body.
3. Gas Exchange
Gas exchange occurs at the Alveoli level:
- Oxygen Transport:
- Oxygen diffuses from the alveoli into the capillaries and binds to hemoglobin in red blood cells.
- Carbon Dioxide Removal:
- Carbon dioxide diffuses from the blood into the alveoli to be exhaled.
4. Regulation of Breathing
The Medulla Oblongata in the brain controls the rate and depth of breathing.
- Chemoreceptors in the blood detect changes in oxygen, carbon dioxide, and pH levels.
- Breathing rate increases when carbon dioxide levels are high.
5. Respiratory Disorders
Common disorders include:
- Asthma: Inflammation and narrowing of airways, causing difficulty in breathing. Asthma
- Bronchitis: Inflammation of the bronchial tubes, leading to mucus buildup. Bronchitis
- Pneumonia: Infection of the lungs, causing alveoli to fill with fluid. Pneumonia
- Emphysema: Damage to alveoli, reducing gas exchange efficiency. Emphysema
6. Importance of the Respiratory System
The Human Respiratory System is essential for:
- Providing oxygen for cellular respiration.
- Removing waste products like carbon dioxide.
- Maintaining the body’s pH balance.
- Supporting speech and smell.
Conclusion
Understanding the Human Respiratory System is crucial to appreciating how the body maintains oxygen supply and removes waste gases. By studying its structure, functions, and associated disorders, we gain insights into maintaining respiratory health and addressing potential issues effectively.
This lecture includes important links for further reading on each component and processes.

Cell : Organelles And Their Functions

Introduction:
Good day, everyone. Today, we are going to discuss the structural organisation of a cell, which is the basic unit of life. According to the NCERT curriculum in the chapter titled “The Fundamental Unit of Life“, the cell is the smallest functional and structural unit of an organism, capable of performing all life processes. All living organisms, from the simplest bacteria to complex multicellular organisms like humans, are made up of cells. Let’s delve into the details of cellular organisation, its structure, and the functions of its components.
1. Discovery of the Cell:
- Robert Hooke discovered dead cells in a thin slice of cork under a primitive or old microscope in 1665.
- Anton van Leeuwenhoek discovered living cells in the pond water by using an improved microscope in 1674.
- Robert Brown discovered and named the cell nucleus in 1831.
- The Cell Theory, proposed by Matthias Schleiden and Theodor Schwann in 1838-39, states that:
- All living organisms are composed of cells.
- The cell is the basic unit of life.
- All cells arise from pre-existing cells, as expanded or added by Rudolf Virchow in 1855.
2. Types of Cells:
Cells are classified into two main types:
- Prokaryotic Cells: These lack a well-defined nucleus and membrane-bound organelles. Examples include bacteria and archaea.
- Eukaryotic Cells: These cells have a true nucleus and membrane-bound organelles. Examples include plants, animals, fungi, and protists.
Feature | Prokaryotic cell | Eukaryotic cell |
---|---|---|
Nucleus | Absent (Nucleoid region present) | Present (Well-defined, membrane-bound nucleus) |
Size | Smaller (0.1 – 5.0 µm) | Larger (10 – 100 µm) |
Cell Type | Unicellular (mostly) | Multicellular or unicellular |
Organelles | No membrane-bound organelles (e.g., mitochondria, ER) | Membrane-bound organelles (e.g., mitochondria, ER) |
Ribosomes | Smaller (70S type) | Larger (80S type, except in mitochondria and chloroplasts) |
Examples | Bacteria, Archaea | Plants, Animals, Fungi, Protists |
3. Types of Organisms:
There are two types of organisms:
- Unicellular Organisms: Organisms that consist of a single cell. Example: Amoeba, bacteria, Paramoecium, and Chlamydomonas.
- Multicellular Organisms: Organisms with multiple cells. Example: fungi, plants, and animals.
4. Structural Organization of the Cell:
Cells are made up of various structures and organelles, each performing specific functions. Below are the main components of a eukaryotic cell.
-
- Structure: The plasma membrane is a thin, flexible barrier membrane made of a lipid bilayer with embedded proteins.
- Function: Regulates movement of substances in and out of the cell, maintains cell shape, and facilitates communication between cells.
-
Cell Wall (in Plant Cells):
- Structure: Composed of cellulose in plants, found only in plant cells, fungi, and some bacteria.
- Function: Provides structural support and protects the cell.
-
- Structure: The control centre of the cell, surrounded by the nuclear envelope.
- Function: Stores genetic material and controls cell activities.
-
- Structure: A jelly-like fluid filling the cell.
- Function: Site for most cellular processes and reactions.
-
- Structure: A network of membranous tubules and sacs.
- Function: Rough ER aids in protein synthesis, while Smooth ER helps in lipid synthesis and detoxification.
-
- Structure: Flattened sacs.
- Function: Modifies, sorts, and packages proteins and lipids.
-
- Structure: Double-membrane-bound organelles.
- Function: Produce energy (ATP), known as the powerhouses of the cell.
-
- Structure: Small non-membrane-bound structures.
- Function: Sites of protein synthesis.
-
Lysosomes (in Animal Cells):
- Structure: Membrane-bound vesicles containing digestive enzymes.
- Function: Break down cellular waste and foreign substances.
- Structure: Large membrane-bound sacs.
- Function: Store nutrients and maintain turgor pressure in plant cells.
- Plastids (in Plant Cells):
- Structure: Organelles found in plant cells and some algae.
- Function: Chloroplasts are involved in photosynthesis.
- Structure: A network of protein filaments.
- Function: Provides mechanical support and facilitates cell movement.
5. Cell Division and Growth:
- Mitosis: Cell division that results in two genetically identical daughter cells.
- Meiosis: Cell division that results in four genetically diverse cells with half the chromosome number.
6. Functions of a Cell:
The cell is a functional unit of life, performing essential functions for survival, including metabolism, growth, reproduction, response to stimuli, and transportation of substances.
7. Why Do Cells Change States?
The three types of solutions that impact cell behaviour:
- Isotonic Solution: Equal solute concentration as inside the cell, cell remains stable.
- Hypotonic Solution: Lower solute concentration, cell gains water and swells.
- Hypertonic Solution: Higher solute concentration, cell loses water and shrinks.
Conclusion: In short, the cell is a complex yet beautifully organised structure that functions as the foundation of life. Each organelle within the cell has a specific function that contributes to the cell’s overall health and survival. Understanding the structural organisation of the cell helps us appreciate how life works at the microscopic level and how different components come together to form a functional, living unit.
The recent wildfires in Los Angeles
The recent wildfires in Los Angeles have been unprecedented in their scale and impact. The Palisades Fire, which began near the Pacific Palisades neighborhood, rapidly expanded to over 23,000 acres, making it the largest in the city’s history.
Tragically, at least 13 lives have been lost, including that of former actor Rory Sykes. The fires have destroyed more than 12,000 properties and displaced approximately 150,000 residents.
The financial toll is staggering, with damages estimated at $150 billion. The fires have also caused power outages affecting over 250,000 people.
Efforts to combat the fires have been extensive. Governor Gavin Newsom has doubled the deployment of the California National Guard to assist in firefighting and public safety operations. Over 9,000 firefighters, including reinforcements from other states and countries, are on the ground battling the blazes.
The situation remains critical, with strong winds and dry conditions continuing to fuel the fires. Residents in affected areas are urged to follow evacuation orders and stay updated through official channels. For real-time information and resources, the state government has provided a dedicated portal.
For a visual overview of the situation, you can watch the following news coverage: